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課程名稱 |
分子生物學及技術
Molecular Biology and Techniques |
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開課學期 |
109-1 |
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授課對象 |
醫學院 微生物及免疫學組 |
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授課教師 |
詹迺立 |
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課號 |
md&ph5074 |
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課程識別碼 |
420 U4800 |
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班次 |
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學分 |
4.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二9,10(16:30~18:20)星期四9,10(16:30~18:20) |
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上課地點 |
基醫302基醫302 |
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備註 |
本課程中文授課,使用英文教科書。@研究所共同課程。與胡務亮、許秉寧、方偉宏、詹智強、張明富、李明學、余明俊、林敬哲、陳炳宏、張 鑫、鄧述諄、李財坤、詹世鵬、楊宏志、許弘明、蔡幸真、阮麗蓉、劉雅雯、蔡欣祐、張百恩、陳佑宗、陳沛隆合授
總人數上限：186人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1091mdph5074_ |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
. |
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課程目標 |
讓研究基礎醫學的研究生，瞭解原核生物與真核細胞內，核酸與蛋白質等巨分子的結構與功能，以奠定其醫學研究的基礎，並讓學生熟悉目前重要的分子生物學相關技術及其在醫學研究上的應用。 |
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課程要求 |
待補 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
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參考書目 |
Molecular Biology of the Gene (7th Edition), by J. D. Watson et al., 2014
Current Protocols in Molecular Biology, by Frederick M. Ausubel , online |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
9/15,9/17 |
1. Introduction (詹迺立)
9/15 DNA as store of information
Major experiments that led to the acceptance of DNA as the genetic material
2. The structure of DNA and RNA (李財坤)
9/17 Structure of nucleic acid, base pairing, DNA forms
Denaturing and renaturing of DNA
High-order DNA structure
DNA topology: supercoiled DNA and topoisomerase
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第2週 |
9/22,9/24 |
3. Genome
9/22 Content, structure, and organization (林敬哲)
9/24 Chromatin and nucleosome (蔡幸真) |
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第3週 |
9/29,10/01 |
4. DNA replication (張鑫)
9/29 Prokaryotic system |
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第4週 |
10/06,10/08 |
10/06 Eukaryotic system (張鑫)
5. Chromosomal dynamics
10/08 The mutability and repair of DNA (方偉宏) |
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第5週 |
10/13,10/15 |
10/13 Homologous recombination
Site-specific recombination and transposition (鄧述諄)
6-1. Transcription, regulation and processing
10/15 Mechanism of transcription (許弘明) |
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第6週 |
10/20,10/22 |
10/20 Transcriptional regulation in prokaryotes (許弘明)
10/22 第一次期中考 (考試範圍9/15~10/20) |
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第7週 |
10/27,10/29 |
6-2. Transcription, regulation and processing (阮麗蓉)
10/27 Transcriptional regulation in eukaryotes
10/29 Epigenetic regulation |
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第8週 |
11/03,11/05 |
7. RNA splicing
11/03 RNA splicing (詹世鵬)
8. Translation
11/05 Assembly line for protein synthesis (張明富)
Transfer RNA is the translated adaptor |
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第9週 |
11/10,11/12 |
11/10 Ribosomes provide a translation factory (張明富)
Codons and their significance
Translation in cell free system
9. Post-transcriptional regulation
10. Regulatory RNAs
11/12 Post-transcriptional regulation (蔡欣祐)
Regulatory RNAs
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第10週 |
11/17,11/19 |
11. Genes in the development
11/17 Gene and development (張百恩)
12. Genes in tumorigenesis
11/19 Oncogenes (李明學)
Tumor suppressor genes |
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第11週 |
11/24,11/26 |
11/24 Immune diversity and immunotherapy (許秉寧)
11/26 第二次期中考(考試範圍 10/27~11/24) |
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第12週 |
12/01,12/03 |
13. Recombinant DNA technology
12/01 DNA cloning and manipulation (陳炳宏)
PCR
Construction of cDNA and genomic libraries (Colony and plaque hybridization)
Differential display
Site-direct mutagenesis
CRISPR genome editing
DNA synthesis/synthetic biology
12/03 Amplification and/or target enrichment of the genome (陳沛隆)
Genetic and epigenetic variants (and various methods to detect them, including Southern, Northern and Western blotting)
Microarray
Sanger sequencing and next-generation sequencing (NGS)
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第13週 |
12/08,12/10 |
12/08 Phage strategies: control of lytic and lysogenic pathway (楊宏志)
Phage display library
A brief introduction to animal viruses
14. Molecular Systems Biology and Bioinformatics
12/10 Molecular Systems Biology and Bioinformatics (余明俊) |
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第14週 |
12/15,12/17 |
15. Gene therapy
12/15 Viral vectors (胡務亮)
Clinical application
16. Model organisms
12/17 Yeast (劉雅雯) |
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第15週 |
12/22,12/24 |
12/22 C. elegans (詹世鵬)
12/24 Drosophila (詹智強) |
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第16週 |
12/29,12/31 |
12/29 Transgenic mice (陳佑宗)
12/31 期末考 (考試範圍 12/01~12/29，修習分子生物學及技術者須參加考試) |
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